Nucleic acid sequence identification
US7001721B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 20, 1999 |
| Grant date | Feb 21, 2006 |
| Priority date | — |
| Expiry date | May 20, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/658
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed are methods for determining the presence or absence of a target nucleic acid (e.g. DNA) sequence in a sample nucleic acid, the method comprising: (a) exposing the sample to a detection agent comprising a colloid metal surface associated with a SER(R)S active species (SAS) such as an azo dye and with a target binding species (TBS) which may be PNA which is complementary to the target, and (b) observing the sample agent mixture using SER(R)S to detect any surface enhancement of the label wherein the binding of the TBS to the target sequence causes surface enhancement SAS. The detection agent may be exposed to the sample in step (a) as two or more separate components and will generally comprise a first agent and a second agent each having a different TBS, each TBS being capable of binding to the target sequence, and wherein the binding of the first and second TBS to the target sequence brings a metal surface associated with each TBS into proximity thereby causing surface enhancement of an SAS associated with one or both of the metal surfaces.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.