Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
US7001724B1 · kind B1 · utility
113Cited by
11References
47Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Nov 28, 2000 |
| Grant date | Feb 21, 2006 |
| Priority date | — |
| Expiry date | Feb 21, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1006
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides compositions and methods for releasing and for isolating nucleic acids from biological samples, preferably from whole tissue, using cationic surfactants and proteases. The surfactant-protease combinations, when used with whole tissue, macerate the tissue, lyse individual cells, release nucleic acids, and inactivate nucleases. Kits for isolating nucleic acids from biological samples, particularly from whole tissue, are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.