Serum free cultivation of primate embryonic stem cells
US7005252B1 · kind B1 · utility
114Cited by
7References
14Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Mar 9, 2000 |
| Grant date | Feb 28, 2006 |
| Priority date | — |
| Expiry date | Mar 9, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/115
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are methods for culturing primate embryonic stem cells. These cells are cultured on a prolonged and stable basis in the presence of exogenously supplied fibroblast growth factor and in the absence of animal serum. Preferably there is also a fibroblast feeder layer. Also disclosed is a culture media containing fibroblast feeder layer and the fibroblast growth factor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.