Oligonucleotides for nucleic acid amplification and for the detection of Mycobacterium tuberculosis
US7009041B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 6, 1995 |
| Grant date | Mar 7, 2006 |
| Priority date | — |
| Expiry date | Jun 6, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/35
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method, composition and kit for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies using a mixture of blocked and unblocked primers and/or promoter-primers to initiate DNA and RNA synthesis, preferably with reduced non-specific product formation. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.