Single particle electrochemical sensors and methods of utilization
US7022287B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 8, 2002 |
| Grant date | Apr 4, 2006 |
| Priority date | — |
| Expiry date | Mar 9, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/22
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention discloses an electrochemical device for detecting single particles, and methods for using such a device to achieve high sensitivity for detecting particles such as bacteria, viruses, aggregates, immuno-complexes, molecules, or ionic species. The device provides for affinity-based electrochemical detection of particles with single-particle sensitivity. The disclosed device and methods are based on microelectrodes with surface-attached, affinity ligands (e.g., antibodies, combinatorial peptides, glycolipids) that bind selectively to some target particle species. The electrodes electrolyze chemical species present in the particle-containing solution, and particle interaction with a sensor element modulates its electrolytic activity. The devices may be used individually, employed as sensors, used in arrays for a single specific type of particle or for a range of particle types, or configured into arrays of sensors having both these attributes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.