Methods for attaching proteins to lipidic microparticles with high efficiency

US7022336B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Demetrios Papahadjopoulos · San Francisco, US
• Keelung Hong · Mill Valley, US
• Weiwen Zheng · San Francisco, US
• Dmitri B. Kirpotin · San Francisco, US

Key dates

• Filing date: May 17, 2004
• Grant date: Apr 4, 2006
• Expiry date: May 17, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/88
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention provides methods of attaching proteins to lipidic microparticles with efficiencies of at least 77%. The proteins are attached to linker molecules comprising a hydrophilic domain and a hydrophobic domain. The proteins can be antibodies, antibody fragments, hormones, growth factors, enzymes, or nucleic acid binding proteins, or other proteins. The proteins can be chemically conjugated to the linker molecules, or they can be fused to the linker molecules by recombinant techniques.