Topoisomerase-based reagents and methods for molecular cloning
US7026141B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 7, 2003 |
| Grant date | Apr 11, 2006 |
| Priority date | — |
| Expiry date | Feb 22, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2710/24122
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
This invention provides a modified vaccinia topoisomerase enzyme containing an affinity tag which is capable of facilitating purification of protein-DNA complexes away from unbound DNA. This invention further provides a modified sequence specific topoisomerase enzyme.This invention provides a method of ligating duplex DNAs, a method of molecular cloning of DNA, a method of synthesizing polynucleotides, and a method of gene targeting.Lastly, this invention provides a recombinant DNA molecule composed of segments of DNA which have been joined ex vivo by the use of a sequence specific topoisomerase and which has the capacity to transform a suitable host cell comprising a DNA sequence encoding polypeptide activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.