Natural promoters for gene expression in C1 metabolizing bacteria
US7026464B2 · kind B2 · utility
11Cited by
2References
3Claims
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Key dates
| Filing date | Oct 20, 2003 |
| Grant date | Apr 11, 2006 |
| Priority date | — |
| Expiry date | Feb 19, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/195
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Genes have been identified in the Methylomonas genome that are responsive to various metabolic and growth conditions. The identified responsiveness of these genes allows for the use of their promoters in regulated gene expression in C1 metabolizing bacteria. In particular, the hps promoter, which in its native state drives the expression of 3-hexulose-6-phosphate synthase (HPS), was found to be useful for directing expression of heterolgous coding regions (e.g., crtZ) in the obligate methanotroph Methylomonas sp. 16a.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.