Process and equipment for plasmid purification
US7026468B2 · kind B2 · utility
9Cited by
29References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 29, 2001 |
| Grant date | Apr 11, 2006 |
| Priority date | — |
| Expiry date | Sep 8, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1017
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A scalable alkaline lysis process, including procedures and devices for the isolation of large quantities (grams and kilograms) of plasmid DNA from recombinant E. coli cell. Effective, contgrollable, and economical operation, and consistent low level of host chromosomal DNA in the final plasmid product. Involves a series of new unit operations and devices for cell resuspension, cell lysis, and nuetralization.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.