Methods for the production of stably-transformed, fertile wheat employing agrobacterium-mediated transformation and compositions derived therefrom
US7026528B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 31, 2002 |
| Grant date | Apr 11, 2006 |
| Priority date | — |
| Expiry date | Sep 16, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8205
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are processes for producing stably transformed fertile wheat a system of transforming wheat via Agrobacterium. This invention provides methods transforming a variety of explants, such as freshly isolated or pre-cultured immature embryos, embryogenic callus and suspension cells. Also disclosed are methods for recovering transgenic plants after transformation within a short period of time, if the explants are regenerable at the time of transformation. Thus the frequency of somaclonal variation associated with prolonged in vitro culture period is significantly reduced. The transformation frequency using this system is comparable to or better than published methods using other systems, such as microprojectile bombardment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.