Method for differentiating rat hepatic stem cells to insulin-producing cells
US7029915B2 · kind B2 · utility
10Cited by
2References
14Claims
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Key dates
| Filing date | Feb 24, 2003 |
| Grant date | Apr 18, 2006 |
| Priority date | — |
| Expiry date | Aug 14, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/34
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Highly purified hepatic stem cells are trans-differentiated into pancreatic endocrine hormone-producing cells by culturing them in vitro in a medium containing high levels of glucose. These trans-differentiated cells express insulin, glucagon, and pancreatic polypeptide, but not hepatocyte protein Hep-par. When stimulated with glucose, these cells synthesize and secrete insulin, a response enhanced by nicotinamide. Transplantation of these trans-differentiated cells into a hyperglycemic animal normalizes blood sugar levels in the animal.
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