Highly sensitive gene detection and localization using in situ branched-DNA hybridization

US7033758B2 · kind B2 · utility

Assignee

• BAYER HEALTHCARE LLC · DE

Inventors

• Daryn Kenny · Moraga, US
• Lu Shen · San Francisco, US
• Vincent P. Antao · Berkeley, US
• Audrey N. Player · Cumberland, US
• Wei CAO · Shanghai, CN

Key dates

• Filing date: Jun 1, 2001
• Grant date: Apr 25, 2006
• Expiry date: Aug 20, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6841
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods are provided for highly sensitive and rapid in situ detection of a nucleic acid analyte of a known sequence. The method employs oligonucleotide probes in a series of optimized steps to amplify a signal and decrease background. Sensitivity is enhanced such that the method can detect as few as 1–2 copies of nucleic acid analyte per sample, the sample containing a cell, tissue or similar biological material. Methods of detecting and identifying the position of the nucleic acid analyte in a cell are also provided.