Method of producing an erythroid cell which is undifferentiated yet capable of expressing a heterologous protein
US7033784B1 · kind B1 · utility
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9Claims
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Key dates
| Filing date | Apr 5, 2000 |
| Grant date | Apr 25, 2006 |
| Priority date | — |
| Expiry date | Apr 5, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5023
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of producing an erythroid cell which is substantially undifferentiated but which is capable of expressing a heterologous protein under the control of a globin promoter thereof, which method comprises maintaining growing uninduced erythroid cells in culture for sufficient period of time that the protein is expressed, and isolating a subclone which expresses said protein. Erythroid cells produced by the method, and methods of detecting the interaction of an insect G-protein coupled receptor with an endogenous signaling cascade of erythroid cells are also described and claimed.
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