High throughput assays for the proteolytic activities of clostridial neurotoxins
US7034107B2 · kind B2 · utility
5Cited by
2References
9Claims
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Key dates
| Filing date | Mar 17, 2004 |
| Grant date | Apr 25, 2006 |
| Priority date | — |
| Expiry date | Mar 17, 2024 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/842
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In this application is described substrates for high-throughput assays of clostridial neurotoxin proteolytic activities. Two types of substrates are described for use in assays for the proteolytic activities of clostridial neurotoxins: (1) modified peptides or proteins that can serve as FRET substrates and (2) modified peptides or proteins that can serve as immobilized substrates. In both types a fluorescent molecules is present in the substrate, eliminating the requirement for the addition of a fluorigenic reagent. The assays described can be readily adapted for use in automated or robotic systems.
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