Truncated form of fibrobacter succinogenes 1,3-1, 4-β-d-glucanase with improved enzymatic activity and thermo-tolerance
US7037696B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 5, 2000 |
| Grant date | May 2, 2006 |
| Priority date | — |
| Expiry date | Sep 5, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01073
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A truncated glucanase with an improved thermal stability and a higher specific enzymatic activity than the wild-type enzyme. The truncated glucanase is obtained by removing a number of amino acid residues from the C-terminal of the wild-type 1,3-1,4-β-D-glucanase of Fibrobacter succinogenes. The removal of the C-terminal amino acid residues can be conducted at the genetic level by modifying the gene encoding for the wild type enzyme using, for example, a PCR-based method. Or, it can also be conducted at the protein level by first producing the wild-type enzyme protein and then subjecting the wild-type protein to certain protease action to remove a portion of its C-terminal.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.