Method of preventing modification of synthetic oligonucleotides
US7038027B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jun 12, 2001 |
| Grant date | May 2, 2006 |
| Priority date | — |
| Expiry date | Dec 8, 2022 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The present invention relates to a method of preventing modification of a synthetic oligonucleotide or oligonucleotide analog during removal of at least one β-cyanoethyl protecting group from the oligonucleotide or oligonucleotide analog. The method involves contacting the oligonucleotide or oligonucleotide analog with a basic solution having at least one acrylonitrile scavenger, such as t-butylamine, at a sufficient temperature and for a sufficient period of time to remove at least one β-cyanoethyl protecting group. The present invention also relates to a method of producing a synthetic oligonucleotide or oligonucleotide analog.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.