Insect cell line
US7041500B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 14, 2003 |
| Grant date | May 9, 2006 |
| Priority date | — |
| Expiry date | May 9, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2720/12322
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
An insect cell line capable of growth in serum-free media and secretion of high levels of recombinant proteins, including virus-like particles, upon infection with recombinant baculoviruses, is provided. Methods involving clonal selection processes, serum-weaning and recombinant protein secretion selection are used to create the insect lines of the invention. This cell line supports replication of baculoviruses, serves as host substrate for baculovirus plaque assays, provides a source of insect proteins, acts as a depot for cell transfection to produce recombinant baculoviruses, and express viral recombinant proteins. Extracellular and intracellular viral recombinant proteins and virus-like particles expressed from this cell line are useful as pharmaceutical compositions, vaccines, or diagnostic reagents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.