Method for contacting fluid components with moieties on a surface
US7045287B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 21, 2001 |
| Grant date | May 16, 2006 |
| Priority date | — |
| Expiry date | Jun 25, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An improved method is provided for conducting chemical or biochemical reactions on a substrate surface in which the reactions take place in an enclosed chamber between components of a fluid and molecular moieties present on an interior surface of the chamber, wherein the improvement involves mixing the fluid during the reactions and maximizing contact between the fluid's components and the entirety of the reactive interior surface by (a) applying centrifugal force to the chamber and simultaneously (b) rotating the chamber about an axis thereof. The improved method is particularly advantageous in the context of a hybridization assay in which molecular components within a sample fluid hybridize to surface-bound molecular probes (e.g., as may be present in a spatially defined and physically addressable array) within a hybridization chamber, and wherein process and device parameters (e.g., sample volume, chamber volume, temperature, number of parts and materials, and the like) are critical. The invention enables use of a very small sample volume without need for a correspondingly small device, minimizes contamination (device components are disposable and the number of parts with which …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.