Bacterial delivery system

US7045336B1 · kind B1 · utility

Assignee

• The United States of America as represented by the Secretary of the Army · US

Inventors

• Arthur Branstrom · Rockville, US
• Donata R. Sizemore · Brentwood, US
• Jerald C. Sadoff · Washington, US

Key dates

• Filing date: Sep 6, 1996
• Grant date: May 16, 2006
• Expiry date: Sep 6, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

We describe a bacterial delivery system for the delivery of DNA and antigens into cells. We constructed an attenuated bacterial vector which enters mammalian cells and ruptures delivering functional plasmid DNA and antigens into the cell cytoplasm. This Shigella vector was designed to deliver DNA to colonic surfaces, thus opening the possibility of oral and other mucosal DNA immunization and gene therapy strategies. The attenuated Shigella is also useful as a vaccine for reducing disease symptoms caused by Shigella.