Stabilized primate lentivirus envelope glycoproteins

US7048929B1 · kind B1 · utility

Assignees

• DANA-FARBER CANCER INSTITUTE, INC. · US
• The Trustees of Columbia University in the City of New York · US

Inventors

• Joseph Sodroski · Medford, US
• Richard Wyatt · Village of La Jolla, US
• Peter Kwong · Washington, US
• Wayne A. Hendrickson · Dobbs Ferry, US
• Michael Farzan · Brookline, US

Key dates

• Filing date: Nov 10, 1998
• Grant date: May 23, 2006
• Expiry date: Nov 10, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/16134
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A modified polypeptide corresponding to an envelope glycoprotein of a primate lentivirus is described. The polypeptide has been modified from the wild-type structure so that it has cysteine amino acid residues introduced to create disulfide bonds, a cavity is filled with hydrophobic amino acids, a Proresidue is introduced at a defined turn structure of the protein, or the hydrophobicity is increased across the interface between different domains, while retaining the overall 3-dimensional structure of a discontinuous conserved epitope of the wild-type protein. Preferably, the polypeptide has more than one of those characteristics. Preferably, the primate lentivirus is HIV, and the protein is HIV-1 gp120.