Purified human erythropoietin receptor protein fragment and antibodies derived therefrom
US7053184B2 · kind B2 · utility
Inventor
Key dates
| Filing date | Jan 30, 1998 |
| Grant date | May 30, 2006 |
| Priority date | — |
| Expiry date | Jan 30, 2018 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/71
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A E. coli recombinant plasmid expressing a fusion protein having the human erythropoietin receptor extracellular domain is disclosed. A purified fusion protein produced from such a vector is also disclosed, the fusion protein having a cleavage site suitable for separating the erythropoietin receptor extracellular domain from the remainder of the fusion protein. Antibodies having specific binding affinity for a purified extracellular domain polypeptide are also disclosed. The purified human erythropoietin receptor fragment polypeptide binds erythropoietin. The articles, compositions and methods of the invention are useful for studying ligand binding to erythropoietin receptor and for quantitating the amounts of erythropoietin receptor, as well as for understanding receptor structure and signal transduction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.