Recombinant aav vectors with AAV5 capsids and AAV5 vectors pseudotyped in heterologous capsids

US7056502B2 · kind B2 · utility

Assignee

• The Trustees of the University of Pennsylvania · US

Inventors

• Markus Hildinger · Pforzheim, DE
• James M. Wilson · Glen Mills, US
• Alberto Auricchio · Napoli, IT

Key dates

• Filing date: Apr 23, 2001
• Grant date: Jun 6, 2006
• Expiry date: Mar 20, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/48
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A pseudotyped rAAV is described, which contains sequences derived from AAV5. A method for producing rAAV pseudotyped with AAV5 capsid is described in which the rep proteins of the serotype or any cross-reactive serotype of the AAV ITRs are utilized. A similar method may be provided to generate a pseudotyped rAAV in which minigenes carrying AAV5 ITRs are packaged in capsids of a heterologous AAV serotype. Also provided are pharmaceutical compositions containing the pseudotyped rAAV and methods of using them for gene delivery.