Detection of binding of charged species using PH- or potential-sensitive probes

US7056653B2 · kind B2 · utility

Assignee

• YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM LTD. · IL

Inventors

• Yechezkel Barenholz · Jerusalem, IL
• Danielle Hirsch-Lerner · Jerusalem, IL
• Rivka Cohen · Jerusalem, IL
• Arie Dagan · Jerusalem, IL
• Shimon Gatt · Jerusalem, IL

Key dates

• Filing date: Feb 8, 2001
• Grant date: Jun 6, 2006
• Expiry date: Feb 8, 2021

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2405/04
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The extent of binding of a moiety, such as a biomolecule, to a surface, where the local environment at the surface has a pH or surface potential which is altered upon binding of the moiety, is determined by stably incorporating at the surface a probe which contains a pH- or potential-sensitive fluorophore.