Method of identifying an open reading frame using a nucleic acid molecule encoding multiple start codons and histidine tags
US7056672B2 · kind B2 · utility
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Key dates
| Filing date | Jan 27, 2004 |
| Grant date | Jun 6, 2006 |
| Priority date | — |
| Expiry date | Sep 6, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/21
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes, in each of the three possible reading frames, an ATG start codon and a histidine tag, and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences of interest 5′ or 3′ to the 3-frame His-tag DNA sequence. Vectors also are provided with cloning sites for inserting nucleotide sequences of interest 3′ of the ATG start codon and 5′ of the 3-frame His-tag DNA sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.