Early stage multipotential stem cells in colonies of bone marrow stromal cells

US7056738B2 · kind B2 · utility

Assignees

• Tulane University · US
• Philadelphia Heath and Education Corporation · US

Inventors

• Darwin J. Prockop · Princeton, US
• David C. Colter · Hillsborough Township, US
• Ichiro Sekiya · Tokyo, JP

Key dates

• Filing date: Mar 23, 2001
• Grant date: Jun 6, 2006
• Expiry date: Feb 25, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N5/0663
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Marrow stromal cells (MSCS) are adult stem cells from bone marrow that can differentiate into multiple non-hematopoietic cell lineages. Colonies of human MSCs were shown to contain both small, rapidly self-renewing stem cells (RS cells) and large, more mature cells (mMSCs). Samples enriched for RS cells had a greater potential for multipotential differentiation than samples enriched for mMSCs. Also, RS cells have a series of surface epitopes and expressed proteins that can be used to differentiate RS cells from mMSCs. The results suggest that it will be important to distinguish the two major sub-populations of MSCs in defining their biology and their potentials for cell and gene therapy.