Methods for transforming immature maize embryos
US7057089B2 · kind B2 · utility
24Cited by
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11Claims
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Key dates
| Filing date | Nov 13, 2001 |
| Grant date | Jun 6, 2006 |
| Priority date | — |
| Expiry date | Aug 25, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8207
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for transforming freshly isolated, immature maize embryos and for producing transgenic maize plants. The methods comprise obtaining immature embryos from a maize plant, contacting the embryos with an auxin-depleted or phytohormone-depleted transformation support medium and introducing a nucleotide construct into cells from the embryos prior to subjecting the embryos to conditions which promote embryogenic-tissue formation. The methods additionally comprise identifying or selecting transformed cells and regenerating such cells into transformed maize plants.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.