Electrophoresis gel and gel-forming apparatus
US7060172B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 9, 1999 |
| Grant date | Jun 13, 2006 |
| Priority date | — |
| Expiry date | Apr 9, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44704
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The apparatus includes a container (20) having a base (22) and sides (32) adapted to receive a plurality of plastic gel cassettes. An inlet port (12) is positioned in the base of the container and in fluid communication with the chamber and a baffle (11) is positioned over the inlet port, such that, in use, when gel forming fluid passes through the inlet port into the chamber, the baffle substantially reduces fluid turbulence and vertical fluid movement in the vicinity of the inlet port during flow of the fluid into the chamber. Pretreatment of the plastic cassette to remove polymerisation inhibitors prior to filling the same with fluid is by exhaustive vacuum treatment, optionally with nitrogen gas purging. This can be achieved conveniently using a vacuum chamber in which one or more plastic cassettes are placed. Optionally the vacuum chamber may be the container in which the cassettes are filled with fluid. No barrier films or chemical scavengers are required.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.