Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling
US7060441B2 · kind B2 · utility
21Cited by
7References
31Claims
0Family size
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Key dates
| Filing date | May 3, 2002 |
| Grant date | Jun 13, 2006 |
| Priority date | — |
| Expiry date | Mar 14, 2024 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A single- or double-stranded deoxyribonucleic acid (DNA) is fragmented and labeled by chemically fragmenting the DNA by creating at least one abasic site on the DNA, and attaching a marker to at least one of the fragments by means of a labeling reagent, the reagent covalently and predominantly coupling to at least one phosphate of the fragment. This method can be applied in the field of diagnosis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.