Protein localization assays for toxicity and antidotes thereto
US7060447B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 13, 2003 |
| Grant date | Jun 13, 2006 |
| Priority date | — |
| Expiry date | Nov 13, 2023 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/12
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Intracellular translocation of proteins, particularly protein kinase C (PKC) isoenzymes, provides a surrogate test system for determining toxicity of candidate compounds. The profile of translocation with respect to at least one and preferably two or more signal transduction proteins can be correlated with-that of known toxins. In addition, databases of such profiles with respect to toxins of various types provide a useful set of standards for evaluating toxicity of candidate compounds. Moreover, to the extent that a toxin's profile mimics that found in a diseased state, the toxin can be used to construct screens for compounds alleviating the disease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.