Method for reconstituting a recombinant protein to its biologically active form

US7060460B2 · kind B2 · utility

Assignee

• BOEHRINGER INGELHEIM AUSTRIA GMBH · AT

Inventors

• Roman Necina · Wien, AT
• Robert Schlegl · Siegenfeld, AT
• Alois Jungbauer · Wien, AT
• Christine Machold · Wien, AT

Key dates

• Filing date: Oct 2, 2002
• Grant date: Jun 13, 2006
• Expiry date: Oct 2, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K1/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In a method for reconstituting a recombinant protein from a denatured state to its active form, a feed solution containing the recombinant protein in its denatured and/or in biologically inactive intermediate forms is subjected to a chromatographic separation process, in which the protein is reconstituted under conditions that promote refolding of the protein and the intermediate forms are separated from the refolded protein. The denatured form and/or the inactive intermediate forms of the protein are separated from the refolded protein in a continuous or quasi-continuous manner and optionally recycled to the feed solution.