Polycationic water soluble copolymer and method for transferring polyanionic macromolecules across biological barriers
US7060498B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 28, 2001 |
| Grant date | Jun 13, 2006 |
| Priority date | — |
| Expiry date | Jun 28, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC08L2205/05
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present provides a carrier molecule for transporting a polyanioic macromolecule such as a nucleic acid across a biological barrier of a cell. The carrier has a biocompatible backbone polymer with two or more polycationic polymer fragments covalently linked. In one embodiment, the backbone polymer is polyethylene glycol (PEG) and the polycationic polymer is polyethylenimine (PEI). The copolymer carrier molecule can be complexed with a polyanionic macromolecule such as a nucleic acid (NA). The NA/copolymer complex is stable in biological conditions by forming a special coreshell-like micelle structure. The nucleic acid can be rapidly released from the complex when biodegradation linker are used to bind the polycationic polymer fragments to the polymer backbone. The carriers and complexes of the invention can be used tin methods of delivering the polycainonic macromolecules to cells both in vitro and in vivo.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.