Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses
US7060804B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 22, 2001 |
| Grant date | Jun 13, 2006 |
| Priority date | — |
| Expiry date | Jan 22, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5434
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention concerns a method for isolating proteins and/or protein and nucleic acid associations in a sample, comprising steps which consist in: contacting said sample and magnetic colloidal particles comprising a core and a coat wherein: the core is magnetic and is coated with at least a polymer comprising functional groups X selected among amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups or mixtures thereof, whereof at least one fraction has reacted with other functional groups of the coat, and the coat consists of a polymer bearing functional groups Z and Z′, capable of ionisation, identical or different, selected among amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulphide, α-halogenocarbonyl, sulphonic acid, maleimide, isocyanate and isothiocyanate groups to form a mixture; incubating said mixture in predetermined conditions; and separating from the mixture the protein and/or protein and nucleic acid associations complexed on the colloidal particles by applying a magnetic field. The invention also concerns a complex consisting of colloidal particles and proteins, a reagent comprising…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.