Methods of making pro-urokinase mutants
US7070958B2 · kind B2 · utility
2Cited by
6References
24Claims
0Family size
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Key dates
| Filing date | Apr 16, 2004 |
| Grant date | Jul 4, 2006 |
| Priority date | — |
| Expiry date | Jul 13, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/21073
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for producing non-glycosylated, single-chain and two-chain pro-urokinase (pro-UK) mutants. The methods include cultivating a specific E. coli type B strain that has been transformed with specific plasmids carrying a cDNA sequence that encodes pro-UK mutants and carries specific promoter sequences. Products produced by the methods have medical use for thrombolysis performed while sparing hemostatic clots, e.g., for particular applications such as after a stroke or heart attack.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.