Substrate activation kit and method for detecting DNA and the like using the same
US7078172B1 · kind B1 · utility
13Cited by
1References
11Claims
0Family size
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Key dates
| Filing date | Jun 20, 2001 |
| Grant date | Jul 18, 2006 |
| Priority date | — |
| Expiry date | Jul 2, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/552
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
It is intended to provide a method whereby DNA can be conveniently and rigidly immobilized on a substrate at such a high density as achieved by the conventional methods and a method of accurately detecting DNA. A substrate activation kit comprising a phosphate buffer (pH6), a solution A (an aqueous solution) of N-hydroxysuccinimide and a solution B (a dioxide solution) of 1-[3-(dimethylamino) propyl]3-ethylcarbodiimide; and a method of detecting DNA characterized by comprising spotting DNA on a substrate having been activated by using the above activation kit and then hybridizing the spotted DNA with fluorescence labeled DNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.