Chemically modified oligonucleotide for site-directed mutagenesis
US7078389B2 · kind B2 · utility
5Cited by
1References
9Claims
0Family size
Assignee
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Key dates
| Filing date | Feb 14, 2001 |
| Grant date | Jul 18, 2006 |
| Priority date | — |
| Expiry date | Jul 16, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/3511
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A mutagenic, triplex-forming oligonucleotide and methods for use thereof wherein the oligonucleotide is chemically modified to incorporate a mutagen and forms a triple-stranded nucleic acid molecule with a specific DNA segment of a target DNA molecule. Upon formation of the triplex, the mutagen is brought into proximity with the target molecule and causes a mutation at a specific site therein. The mutation activates, inactivates or alters the activity and function of the target molecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.