Nucleotide analogues comprising a reporter moiety and a polymerase enzyme blocking moiety
US7078499B2 · kind B2 · utility
119Cited by
3References
15Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 31, 2001 |
| Grant date | Jul 18, 2006 |
| Priority date | — |
| Expiry date | Aug 25, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Nucleotides comprising a reporter moiety and a polymerase enzyme blocking moiety in which the reporter moiety does not also act as a polymerase enzyme blocking moiety are described. Also described are compounds of Formula (I): wherein W is a phosphate group, B is a base, Y is a linker comprising an enzyme-cleavable group, R2 is a reporter moiety, R3 is selected from H or OH, Z and Z′ are selected from H, OH, or a group X—R1, wherein X is a linker comprising an enzyme-cleavable group and R1 is a polymerase enzyme blocking group, provided that at least one of Z and Z′ is X—R1.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.