Carbonyl reductases, polynucleotides comprising DNA encoding the same, methods for producing the same, and methods for producing optically active alcohol utilizing the same
US7083962B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 15, 2004 |
| Grant date | Aug 1, 2006 |
| Priority date | — |
| Expiry date | Jul 13, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An objective of the present invention is to provide methods for efficiently producing (S)-1-(3,4-dimethoxyphenyl)-2-propanol at a high optical purity. Another objective is to provide novel reductases which reduce 3,4-dimethoxyphenylacetone, using NADPH as a coenzyme, to produce (S)-1-(3,4-dimethoxyphenyl)-2-propanol with a high optical purity.The inventors found that a 3,4-dimethoxyphenylacetone-reducing enzyme present in Torulaspora delbrueckii is a novel carbonyl reductase that reduces various carbonyls. This novel enzyme reduces 3,4-dimethoxyphenylacetone in a reduction reaction to produce (S)-1-(3,4-dimethoxyphenyl)-2-propanol with a high optical purity and at a high yield. Furthermore, the inventors isolated a DNA that encodes the present enzyme, and generated a recombinant bacterium which highly expresses the present enzyme. Thus, the present inventors established a simple and highly economical method of obtaining optically active alcohols with a high optical purity and at a high yield.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.