Patent · US Expired

Method for purifying enzymes from clostridium histolyticum using multi-stage chromatography

US7083964B2 · kind B2 · utility

18Cited by

2References

20Claims

0Family size

Assignee

Nordmark Arzneimittel GmbH & Co. KG · DE

Inventors

Manfred Kurfürst · Moorrege, DE
Stefan Schmidbauer · Lahntal, DE

Key dates

Filing date	Jun 1, 2002
Grant date	Aug 1, 2006
Priority date	—
Expiry date	Nov 4, 2022

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y304/22008
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a method for purifying at least one enzyme obtained in an excess fermentation of Clostridium histolyticum. It is provided for that the enzymes of the excess fermentation are separated by a multistage chromatography method by exclusively using chromatography materials on styrene/divinyl-benzene base and/or on base of in particular ceramic hydroxylapatite.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.