Methods for searching polynucleotide probe targets in databases
US7085652B2 · kind B2 · utility
2Cited by
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27Claims
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Key dates
| Filing date | Feb 14, 2003 |
| Grant date | Aug 1, 2006 |
| Priority date | — |
| Expiry date | Aug 2, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/10
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
Methods for determining the binding affinity of a probe to a target or targets in a polynucleotide composite using an automated system. Such methods determine relative binding sites based on thermodynamic principles, using a thermodynamic alphabet and a thermodynamic scoring matrix, with appropriate computer software, such as BLASTP. The invention also provides methods for designing polynucleotide probes to be used in hybridization assays, and minimizing the occurrence of cross-hybridization.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.