Design, synthesis and use of specific polyamide DNA-binding ligands
US7087378B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 11, 1999 |
| Grant date | Aug 8, 2006 |
| Priority date | — |
| Expiry date | Aug 11, 2019 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention encompasses improved selective polyamides for binding to specific nucleotide sequences of double stranded DNA as well as methods for designing and synthesizing polyamide DNA binding ligands that are selective for an identified specific nucleotide sequence. The 3-hydroxy-N-methylpyrrole/N-methylpyrrole carboxamide pair specifically recognizes the T•A base pair, while the N-methylpyrrole/3-hydroxy-N-methylpyrrole pair recognizes A•T nucleotide pairs. Similarly, an N-methylimidizole/N-methylpyrrole carboxamide pair specifically recognizes the G•C nucleotide pair, and the N-methylpyrrole/N-methylimidizole carboxamide pair recognizes the C•G nucleotide pair.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.