Sequence specific DNA binding by p53

US7087583B2 · kind B2 · utility

Assignees

• THE JOHNS HOPKINS UNIVERSITY · US
• Genzyme Corporation · US

Inventors

• Bert Vogelstein · Baltimore, US
• Kenneth W. Kinzler · Baltimore, US
• Michael I. Sherman · Glens Falls, US

Key dates

• Filing date: Mar 22, 2001
• Grant date: Aug 8, 2006
• Expiry date: Apr 30, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/154
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Specific sequences in the human genome are the sites of strong binding of wild-type p53 protein, but not mutant forms of the protein. These sequences are used diagnostically to detect cells in which the amount of wild-type p53 is diminished. The sequences can also be used to screen for agents which correct for loss of wild-type p53 to DNA in cancer cells.