Derivatized versions of ligase enzymes for constructing DNA sequences
US7090979B2 · kind B2 · utility
1Cited by
6References
10Claims
0Family size
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Key dates
| Filing date | Nov 21, 2003 |
| Grant date | Aug 15, 2006 |
| Priority date | — |
| Expiry date | Jul 19, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of making very long, double-stranded synthetic poly-nucleotides. A multiplicity of short oligonucleotides is provided. The short oligonucleotides are sequentially hybridized to each other. Enzymatic ligation of the oligonucleotides provides a contiguous piece of PCR-ready DNA of predetermined sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.