Patent · US Expired

Protein engineering

US7092825B1 · kind B1 · utility

1Cited by
3References
13Claims
0Family size

Assignee

Inventors

Key dates

Filing dateOct 21, 1999
Grant dateAug 15, 2006
Priority date
Expiry dateOct 21, 2019

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG16B15/00
  • WIPO fieldComputer technology
  • WIPO sectorElectrical engineering

Abstract

A method of protein engineering is provided wherein a searchable computer database is created comprising entries in the form of descriptions of a location and orientation in 3D space of side chains of the constituent amino acid residues of a framework protein. A query is created which corresponds to a description of a location and orientation in 3D space of respective side chains of amino acid residues of a sample protein. The location and orientation in 3D space of constituent side-chains is preferably described as a Cα–Cβ vector. The query is used to search the database and thereby identify a hit which corresponds to a framework protein having structural similarity with said sample protein. Framework protein “hits” so identified may be suitable candidates for further modification. A particular advantage of the present invention is that a modified framework protein may display one or more desired characteristics, such as a function similar to or inhibitory of the sample protein.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.