Methods and compositions for amplification of RNA sequences
US7094536B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Mar 11, 2002 |
| Grant date | Aug 22, 2006 |
| Priority date | — |
| Expiry date | Mar 11, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods for linear and exponential amplification of RNA. They are particularly suitable for amplifying a plurality of RNA species in a sample. The methods are based on hybridization of polynucleotide comprising a propromoter sequence to a primer extension product to generate an intermediate polynucleotide capable of driving transcription, whereby multiple copies of RNA products comprising sequences complementary to an RNA sequence of interest are generated. The methods are useful for preparation of nucleic acid libraries and substrates for analysis of gene expression of cells in biological samples. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.