Use of mixed duplex oligonucleotides to effect localized genetic changes in plants
US7094606B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Aug 5, 1998 |
| Grant date | Aug 22, 2006 |
| Priority date | — |
| Expiry date | Dec 13, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8289
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention concerns the use of duplex oligonucleotides about 25 to 30 base pairs to introduce site specific genetic alterations in plant cells. The oligonucleotides can be delivered by mechanical (biolistic) systems or by electrpoporation of plant protoplasts. Thereafter plants having the genetic alteration can be generated from the altered cells. In specific embodiments the invention concerns alteration in the gene that encode acid invertase, UDP-glucose pyrophosphorylase, polyphenol oxidase, O-methyl transferase, cinnamyl alcohol dehydrogenase, ACC synthase and ACC oxidase or etr-1 or a homolog of etr-1, and plants having isolated point mutations in such genes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.