Solid phase native chemical ligation of unprotected or N-terminal cysteine protected peptides in aqueous solution
US7094871B2 · kind B2 · utility
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20Claims
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Key dates
| Filing date | Nov 15, 2001 |
| Grant date | Aug 22, 2006 |
| Priority date | — |
| Expiry date | Oct 14, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/047
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods, apparatus and kits for synthesizing assembled peptides and proteins on a solid phase with sequential ligation of three or more unprotected peptide segments using chemoselective and mild ligation chemistries in aqueous solution. Also provided are methods of monitoring solid phase sequential ligation reactions using MALDI or electrospray ionization mass spectrometry of reaction products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.