Compositions and methods for detection and isolation of phosphorylated molecules

US7102005B2 · kind B2 · utility

Assignee

• Molecular Probes, Inc. · US

Inventors

• Brian Agnew · Eugene, US
• Joseph Beechem · Eugene, US
• Kyle Gee · Springfield, US
• Richard P. Haugland · Eugene, US
• Jixiang Liu · Eugene, US
• Vladimir Martin · Eugene, US
• Wayne Forrest Patton · Newton, US
• Thomas Steinberg · Eugene, US

Key dates

• Filing date: May 2, 2003
• Grant date: Sep 5, 2006
• Expiry date: Jun 1, 2024

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/4709
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.