Yeast cells engineered to produce pheromone system protein surrogates and uses therefor

US7105309B2 · kind B2 · utility

Assignee

• Cadus Technologies, Inc. · US

Inventors

• Dana M. Fowlkes · Chapel Hill, US
• James Broach · Princeton, US
• John Manfredi · Ossining, US
• Christine Klein · Ossining, US
• Andrew J. Murphy · Croton-on-Hudson, US
• Jeremy Paul · South Nyack, US
• Joshua Trueheart · Concord, US

Key dates

• Filing date: Oct 1, 2002
• Grant date: Sep 12, 2006
• Expiry date: Dec 11, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Yeast cells are engineered to express both a surrogate of a pheromone system protein (e.g., enzymes involved in maturation of α-factor, transporters of a-factor, pheromone receptors, etc.) and a potential peptide modulator of the surrogate, in such a manner that the inhibition or activation of the surrogate affects a screenable or selectable trait of the yeast cells. Various additional features improve the signal-to-noise ratio of the screening/selection system.