Specific and sensitive nucleic acid detection method

US7105318B2 · kind B2 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Christoph Kessler · Munich, DE
• Gerd Haberhausen · Penzberg, DE
• Knut Bartl · Weilheim in Oberbayern, DE
• Henrik Orum · Frederikssund, DK

Key dates

• Filing date: Dec 17, 2002
• Grant date: Sep 12, 2006
• Expiry date: May 16, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6818
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Method for the detection of a nucleic acid comprising the production of a plurality of amplificates of a section of this nucleic acid with the aid of two primers, one of which can bind to a binding sequence A of the nucleic acid and the other can bind to a binding sequence C′ which is complementary to a sequence C which is located in the 3′ direction from A and does not overlap with A, contacting the amplificates with a probe having a binding sequence D which can bind to a sequence B which is located between the sequences A and C or to the complement thereof, and detecting the formation of a hybrid of the amplificate and probe where the sequence located between the binding sequences A and C contains no nucleotides that do not belong to the binding sequence D of the probe or its complement D′.