Method for ligating nucleic acids and molecular cloning
US7109178B2 · kind B2 · utility
9Cited by
7References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 25, 2002 |
| Grant date | Sep 19, 2006 |
| Priority date | — |
| Expiry date | Dec 22, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.